Experimentally Validated Reconstruction and Analysis of a Genome-Scale Metabolic Model of an Anaerobic Neocallimastigomycota Fungus
Abstract
Recent genomic analyses have revealed that anaerobic gut fungi possess both the largest number and highest diversity of lignocellulolytic enzymes of all sequenced fungi, explaining their ability to decompose lignocellulosic substrates, e.g., agricultural waste, into fermentable sugars. Despite their potential, the development of engineering methods for these organisms has been slow due to their complex life cycle, understudied metabolism, and challenging anaerobic culture requirements.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Feb 23, 2021
- Source ID
- 10.1128/msystems.00002-21
Entities
People
- Anna Lipzen
- Bernhard Palsson
- Chris G. Daum
- Christopher E. Lawson
- Igor V. Grigoriev
- Jerry Jenkins
- John K. Henske
- Jonathan M. Monk
- Kerrie W. Barry
- Linda Petzold
- Michael K. Theodorou
- Michelle O'Malley
- Patrick A. Leggieri
- St. Elmo Wilken
- Stephen J. Mondo
- Susanna Seppälä
- Thomas S. Lankiewicz
Organizations
- Army Research Office
- Harper Adams University
- Joint BioEnergy Institute
- Joint Genome Institute
- Lawrence Berkeley National Laboratory
- National Science Foundation
- United States Department of Energy
- University of California, San Diego
- University of California, Santa Barbara