Distinct Bacterial Pathways Influence the Efficacy of Antibiotics against Mycobacterium tuberculosis

Abstract

Understanding how Mycobacterium tuberculosis survives during antibiotic treatment is necessary to rationally devise more effective tuberculosis (TB) chemotherapy regimens. Using genome-wide mutant fitness profiling and the mouse model of TB, we identified genes that alter antibiotic efficacy specifically in the infection environment and associated several of these genes with natural genetic variants found in drug-resistant clinical isolates. These data suggest strategies for synergistic therapies that accelerate bacterial clearance, and they identify mechanisms of adaptation to drug exposure that could influence treatment outcome.

Document Details

Document Type
Pub Defense Publication
Publication Date
Aug 25, 2020
Source ID
10.1128/msystems.00396-20

Entities

People

  • Christopher M Sassetti
  • Clare Smith
  • Megan K. Proulx
  • Michelle M Bellerose
  • Richard E. Baker
  • Thomas R. Ioerger

Organizations

  • Department of Computer Science, University of Oxford
  • National Institutes of Health
  • United States Department of Defense
  • University of Massachusetts Medical School

Tags

Fields of Study

  • Biology

Readers

  • Infectious Disease/Epidemiology
  • Molecular and genetic basis of cancer.
  • Oncology

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech