synDNA—a Synthetic DNA Spike-in Method for Absolute Quantification of Shotgun Metagenomic Sequencing
Abstract
The synDNAs designed in this study enable accurate and reproducible measurements of absolute amount and fold changes of bacterial species in complex microbial communities. The method proposed here is versatile and promising as it can be applied to bacterial communities or genomic features like genes and operons, in addition to being easily adaptable by other research groups at a low cost.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Dec 20, 2022
- Source ID
- 10.1128/msystems.00447-22
Entities
People
- Karsten Zengler
- Livia S Zaramela
- Maxwell Neal
- Megan Tjuanta
- Oriane Moyne
Organizations
- National Science Foundation
- United States Army
- United States Department of Energy
- University of California, San Diego