A Gene Cluster That Encodes Histone Deacetylase Inhibitors Contributes to Bacterial Persistence and Antibiotic Tolerance in Burkholderia thailandensis

Abstract

The discovery of antibiotics such as penicillin and streptomycin marked a historic milestone in the 1940s and heralded a new era of antimicrobial therapy as the modern standard for medical treatment. Yet, even in those early days of discovery, it was noted that a small subset of cells (∼1 in 10 5 ) survived antibiotic treatment and continued to persist, leading to recurrence of chronic infection. These persisters are phenotypic variants that have modified their physiology to survive environmental stress. In this study, we have performed three transcriptomic screens to identify persistence genes that are common between three different stressor conditions. In particular, we identified genes that function in the synthesis of secondary metabolites, small molecules, and complex lipids, which are likely required to maintain the persistence state. Targeting universal persistence genes can lead to the development of clinically relevant antipersistence therapeutics for infectious disease management.

Document Details

Document Type
Pub Defense Publication
Publication Date
Feb 11, 2020
Source ID
10.1128/msystems.00609-19

Entities

People

  • Cheryl D. Gleasner
  • Elizabeth Hong-geller
  • Judith R. Mourant
  • Migun Shakya
  • Nileena Velappan
  • Patrick S. G. Chain
  • Samantha H. Adikari
  • Sofiya N. Micheva-viteva

Organizations

  • Defense Threat Reduction Agency
  • Los Alamos National Laboratory

Tags

Fields of Study

  • Biology

Readers

  • Microbial Pathology
  • Molecular and Cellular Biology
  • Molecular and genetic basis of cancer.