Quantifying Live Microbial Load in Human Saliva Samples over Time Reveals Stable Composition and Dynamic Load
Abstract
Human microbiomes are dynamic ecosystems often composed of hundreds of unique microbial taxa. To detect fluctuations over time in the human oral microbiome, we developed a novel workflow to quantify live microbial cells with flow cytometry in parallel with next-generation sequencing, and applied this method to over 150 unstimulated, timed saliva samples.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Feb 23, 2021
- Source ID
- 10.1128/msystems.01182-20
Entities
People
- Clarisse Marotz
- James T. Morton
- Joanna Coker
- Karsten Zengler
- Pedro Belda-ferre
- Perris Navarro
- Rob Knight
Organizations
- Army Research Office
- Flatiron Institute
- National Institute of Dental and Craniofacial Research
- University of California, San Diego