Immunization with Recombinant Accessory Protein-Deficient SARS-CoV-2 Protects against Lethal Challenge and Viral Transmission

Abstract

Live-attenuated vaccines (LAVs) are able to induce robust immune responses, including both humoral and cellular immunity, representing a very promising option to provide broad and long-term immunity. To develop LAVs for SARS-CoV-2, we engineered attenuated recombinant SARS-CoV-2 (rSARS-CoV-2) that simultaneously lacks the viral open reading frame 3a (ORF3a) in combination with either ORF6, ORF7a, or ORF7b (Δ3a/Δ6, Δ3a/Δ7a, and Δ3a/Δ7b, respectively) proteins.

Document Details

Document Type
Pub Defense Publication
Publication Date
Jun 15, 2023
Source ID
10.1128/spectrum.00653-23

Entities

People

  • Amit Kapoor
  • Andreu Garcia-vilanova
  • Anna Allué-guardia
  • Chengjin Ye
  • James J. Kobie
  • Jordi B. Torrelles
  • Jun-gyu Park
  • Kevin Chiem
  • Luis Martinez-Sobrido
  • Mark R. Walter
  • Paula Pino Tamayo
  • Piyush Dravid
  • Richard K Plemper
  • Vinay Shivanna

Organizations

  • Georgia State University
  • Texas Biomedical Research Institute
  • University of Alabama at Birmingham

Tags

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular Genetics
  • Virology (or Medical Virology).

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech