Communication—Microfluidic Electrochemical Acetylcholine Detection in the Presence of Chlorpyrifos
Abstract
An amperometric acetylcholine sensor was developed for use in a microfluidic system and characterized with chlorpyrifos and its metabolite, chlorpyrifos oxon. This enzymatic sensor was highly selective for acetylcholine, with a detection limit of 0.2 μM and sensitivity of 1.7 nA μM−1from 1–150 μM. Though chlorpyrifos had no effect on sensor function, chlorpyrifos oxon significantly inhibited response across a range of concentrations (0.5–50 μM). Inhibition was reversed by 2-pyridine aldoxime methyl chloride. This platform can be used to both quantify acetylcholine in the presence of chlorpyrifos and as a biorecognition method for chlorpyrifos oxon.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 01, 2019
- Source ID
- 10.1149/2.0711916jes
Entities
People
- David Cliffel
- Dusty R. Miller
- Ethan S. McClain
Organizations
- Intelligence Advanced Research Projects Activity