Total Internal Reflection of Two Lasers in a Single Planar Optical Waveguide
Abstract
Multiplex biomarker quantitation is ideal for tracking the progression of a disease, but sensitive, specific, and quantitative multiplex biosensing remains challenging. We achieved total internal reflection with two lasers in a single planar optical waveguide by coupling 532 nm laser light into the diffraction grating of a waveguide and 635 nm laser light directly into the thin film of the same waveguide. We confirmed that the evanescent fields generated in the waveguide can excite two fluorescent dyes (Alexa Fluor 532 and Alexa Fluor 647) held to the surface of the waveguide through biotin-streptavidin chemistry. This sensing concept holds significant potential as a platform for multiplex biomarker detection.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- May 03, 2022
- Source ID
- 10.1149/2754-2726/ac6523
Entities
People
- Harshini Mukundan
- Kiersten D. Lenz
- Philip A. Kocheril
Organizations
- United States Department of Defense