Neuromotor deficits and altered physiological responses to repeated exertional heat stroke exposures in mice
Abstract
Exertional heat stroke (EHS) is a life-threatening illness that can lead to negative health outcomes. Using a “severe” preclinical mouse model of EHS, we tested the hypotheses that one EHS exposure results in altered susceptibility to a subsequent EHS and reduced neuromotor performance. Female C57BL/6 mice underwent two protocols, 2 wk apart, either an EHS trial (EHS) or a sham exercise control trial (EXC). For EHS, mice ran in a forced running wheel at 37.5°C/40% relative humidity until loss of consciousness, followed by a slow cooling protocol (2 h recovery at 37.5°C). EXC mice exercised equally but in ∼22°C. Mice were randomized into three groups: 1) EXC-EXC (two consecutive EXC, n = 6, 2) EHS-EXC (EHS followed by EXC, n = 5), and 3) EHS-EHS (repeated EHS, n = 9). Mice underwent noninvasive neuromotor and behavioral tests during recovery and isolated soleus force measurements at the end of recovery. At the first EHS, mice reached average peak core temperatures (Tc,max) of 42.4°C, (46% mortality). On the second EHS, average Tc,max was reduced by ∼0.7°C ( P c,max, and greater deficits in neuromotor and behavioral function during recovery.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Dec 01, 2022
- Source ID
- 10.1152/ajpregu.00152.2022
Entities
People
- Bryce J. Gambino
- Christian K. Garcia
- Jamal M. Alzahrani
- Kevin J. Cusack
- Kevin O Murray
- Laila H. Sheikh
- Orlando Laitano
- Thomas L Clanton
Organizations
- United States Army
- University of Florida