Ultrabright plasmonic fluor nanolabel-enabled detection of a urinary ER stress biomarker in autosomal dominant tubulointerstitial kidney disease
Abstract
Autosomal dominant tubulointerstitial kidney disease (ADTKD)-uromodulin ( UMOD) is an underdiagnosed cause of chronic kidney disease (CKD). Lack of ultrasensitive bioanalytical tools has hindered the discovery of low abundant urinary biomarkers in ADTKD. Here, we developed an ultrasensitive plasmon-enhanced fluorescence-linked immunosorbent assay (p-FLISA). p-FLISA demonstrated that secreted immunoglobulin heavy chain-binding protein is an early urinary endoplasmic reticulum stress biomarker in ADTKD- UMOD, which will be valuable in monitoring disease progression and the treatment response in ADTKD.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 01, 2021
- Source ID
- 10.1152/ajprenal.00231.2021
Entities
People
- Anthony J Bleyer
- Bryce G. Johnson
- Chuang Li
- Jeremiah J Morrissey
- Jeremy S. Duffield
- Kendrah Kidd
- Srikanth Singamaneni
- Stanislav Kmoch
- Yeawon Kim
- Ying Maggie Chen
- Yixuan Wang
- Zheyu Wang
Organizations
- Charles University
- National Institute of Diabetes and Digestive and Kidney Diseases
- Pfizer
- United States Department of Defense
- Wake Forest School of Medicine
- Washington University School of Medicine
- Washington University in St. Louis