Comparison of the Triage Micro Parasite Panel and Microscopy for the Detection ofEntamoeba histolytica/Entamoeba dispar,Giardia lamblia, andCryptosporidium parvumin Stool Samples Collected in Kenya
Abstract
Entamoeba histolytica,Giardia lamblia, andCryptosporidium parvumare three of the most important parasitic causes of acute diarrhea worldwide. Laboratory diagnosis of these parasites is usually done by ova and parasite examination (O&P examination) via microscopy. The sensitivity and specificity of O&P examination varies among laboratories and can be labor intensive and time consuming. The Triage Micro Parasite Panel (BioSite, San Diego, California) is an enzyme immunoassay kit that can detectE. histolytica/E. dispar,G. lamblia, andC. parvumsimultaneously using fresh or frozen stool. The present study evaluated the Triage Micro Parasite Panel in detectingE. histolytica/E. dispar,G. lamblia, andC. parvumcompared to O&P examination in 266 stool samples collected at medical facilities in Kenya. The sensitivity and specificity results for the Triage Micro Parasite Panel were: forE. histolytica/E. dispar: 100%, 100%,G. lamblia: 100%, 100% andC. parvum: 73%, 100%. There was no evidence of cross reactivity using the kit with other parasites identified in the stool specimens. These results indicate that the Triage Micro Parasite Panel is a highly sensitive kit that can be used for screening purposes in large scale studies or outbreak investigations or as a possible alternative to O&P examination.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 01, 2012
- Source ID
- 10.1155/2012/564721
Entities
People
- Brett Swierczewski
- Cliff Odhiambo
- Edwin Oaks
- Elizabeth Odundo
- Janet Ndonye
- Ronald Kirera
Organizations
- United States Department of Defense
- Walter Reed Army Institute of Research