AnIn VitroComparison of PMMA and Calcium Sulfate as Carriers for the Local Delivery of Gallium(III) Nitrate to Staphylococcal Infected Surgical Sites
Abstract
Antibiotic-loaded bone cements, including poly(methyl methacrylate) (PMMA) and calcium sulfate (CaSO4), are often used for treatment of orthopaedic infections involvingStaphylococcusspp., although the effectiveness of this treatment modality may be limited due to the emergence of antimicrobial resistance and/or the development of biofilms within surgical sites. Gallium(III) is an iron analog capable of inhibiting essential iron-dependent pathways, exerting broad antimicrobial activity against multiple microorganisms, includingStaphylococcusspp. Herein, we evaluated PMMA and CaSO4as carriers for delivery of gallium(III) nitrate (Ga(NO3)3) to infected surgical sites by assessing the release kinetics subsequent to incorporation and antimicrobial activity againstS. aureusandS. epidermidis. PMMA and to a lesser extent CaSO4were observed to be compatible as carriers for Ga(NO3)3, eluting concentrations with antimicrobial activity against planktonic bacteria, inhibiting bacterial growth, and preventing bacterial colonization of beads, and effective against established bacterial biofilms ofS. aureusandS. epidermidis. Collectively, ourin vitroresults indicate that PMMA is a more suitable carrier compared to CaSO4for delivery of Ga(NO3)3; moreover they provide evidence for the potential use of Ga(NO3)3with PMMA as a strategy for the prevention and/or treatment for orthopaedic infections.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 01, 2016
- Source ID
- 10.1155/2016/7078989
Entities
People
- Carlos J. Sanchez
- David Chang
- David J. Tennent
- Joseph C Wenke
- Rebecca A. Garcia
Organizations
- United States Army Medical Research and Development Command