Resonance Raman Spectra for the In Situ Identification of Bacteria Strains and Their Inactivation Mechanism
Abstract
The resonance Raman spectra of bacterial carotenoids have been employed to identify bacterial strains and their intensity changes as a function of ultraviolet (UV) radiation dose have been used to differentiate between live and dead bacteria. In addition, the resonance-enhanced Raman spectra enabled us to detect bacteria in water at much lower concentrations (∼108 cells/mL) than normally detected spectroscopically. A handheld spectrometer capable of recording resonance Raman spectra in situ was designed, constructed, and was used to record the spectra. In addition to bacteria, the method presented in this paper may also be used to identify fungi, viruses, and plants, in situ, and detect infections within a very short period of time.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Feb 19, 2021
- Source ID
- 10.1177/0003702821992834
Entities
People
- Anushka Nagpal
- Dinesh Dhankhar
- Jie Chen
- Peter M. Rentzepis
- Runze Li
- Thomas C. Cesario
Organizations
- Air Force Office of Scientific Research
- Robert A. Welch Foundation
- Shanghai Jiao Tong University
- Texas A&M University
- Texas Engineering Experiment Station
- University of California, Irvine