Effect of iron and silica nanoparticles’ size on in vitro human skin binding and penetration
Abstract
This in vitro skin study determined absorption, diffusion, and binding rates of four [14C]-labeled nanoparticles (NPs): 12 nm Fe3O4, 32 nm Fe3O4@SiO2, 33 nm SiO2, and 78 nm SiO2 in each layer of human cadaver skin. In vitro microdialysis device and flow-through skin diffusion system were used to measure the binding affinity to the stratum corneum (SC) and permeability into/through skin layer of the four NPs with different physical–chemical properties, respectively, in short (30 min) and/or long (24 hours) exposures. Results show that NP size is an important factor affecting NP percutaneous absorption. The 12 nm Fe3O4 NPs reached the SC and viable epidermis; 32 nm Fe3O4@SiO2 core/shell NPs only reached SC. However, 33 nm and 78 nm silica NPs did not permeate SC. Similar patterns were observed for NP binding affinity to SC and dermatopharmacokinetic analysis using the tape stripping method. The binding affinity determination may be a useful method to efficiently screen skin penetration of NPs.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 01, 2019
- Source ID
- 10.1177/2397847319893054
Entities
People
- Alissa Zhang
- Bruce A Buchholz
- Eui Jung
- Hanjiang Zhu
- Howard Maibach
- Wei Wang
- Xiaoying Hui
Organizations
- Defense Threat Reduction Agency
- Lawrence Livermore National Laboratory
- Saudi Aramco
- University of California, San Francisco