Comparative analysis of differentially abundant proteins quantified by LC–MS/MS between flash frozen and laser microdissected OCT-embedded breast tumor samples
Abstract
Proteomic studies are typically conducted using flash-frozen (FF) samples utilizing tandem mass spectrometry (MS). However, FF specimens are comprised of multiple cell types, making it difficult to ascertain the proteomic profiles of specific cells. Conversely, OCT-embedded (Optimal Cutting Temperature compound) specimens can undergo laser microdissection (LMD) to capture and study specific cell types separately from the cell mixture. In the current study, we compared proteomic data obtained from FF and OCT samples to determine if samples that are stored and processed differently produce comparable results.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Nov 07, 2020
- Source ID
- 10.1186/s12014-020-09300-y
Entities
People
- Albert J. Kovatich
- Brenda Deyarmin
- Craig D Shriver
- Guisong Wang
- Hai Hu
- J. Leigh Fantacone-campbell
- Jeffrey A. Hooke
- Lori A Sturtz
- Mary Lou Cutler
- Michael A Kiebish
- Niven R Narain
- Praveen-Kumar Raj-Kumar
- Punit Shah
- Rangaprasad Sarangarajan
- Richard Searfoss
Organizations
- United States Department of Defense