Genetic voltage indicators
Abstract
As a “holy grail” of neuroscience, optical imaging of membrane potential could enable high resolution measurements of spiking and synaptic activity in neuronal populations. This has been partly achieved using organic voltage-sensitive dyes in vitro, or in invertebrate preparations yet unspecific staining has prevented single-cell resolution measurements from mammalian preparations in vivo. The development of genetically encoded voltage indicators (GEVIs) and chemogenetic sensors has enabled targeting voltage indicators to plasma membranes and selective neuronal populations. Here, we review recent advances in the design and use of genetic voltage indicators and discuss advantages and disadvantages of three classes of them. Although genetic voltage indicators could revolutionize neuroscience, there are still significant challenges, particularly two-photon performance. To overcome them may require cross-disciplinary collaborations, team effort, and sustained support by large-scale research initiatives.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Sep 12, 2019
- Source ID
- 10.1186/s12915-019-0682-0
Entities
People
- Christiane Grimm
- Rafael Yuste
- Victor H Cornejo
- Yuki Bando
Organizations
- National Eye Institute
- National Institute of Neurological Disorders and Stroke
- National Science Foundation
- The Pew Charitable Trusts
- United States Army Research Laboratory