Use of magnetic nanotrap particles in capturing Yersinia pestis virulence factors, nucleic acids and bacteria
Abstract
Many pathogens, including Yersinia pestis, cannot be consistently and reliably cultured from blood. New approaches are needed to facilitate the detection of proteins, nucleic acid and microorganisms in whole blood samples to improve downstream assay performance. Detection of biomarkers in whole blood is difficult due to the presence of host proteins that obscure standard detection mechanisms. Nanotrap® particles are micron-sized hydrogel structures containing a dye molecule as the affinity bait and used to detect host biomarkers, viral nucleic acids and proteins as well as some bacterial markers. Nanotraps have been shown to bind and enrich a wide variety of biomarkers and viruses in clinically relevant matrices such as urine and plasma. Our objective was to characterize the binding ability of Nanotrap particle type CN3080 to Y. pestis bacteria, bacterial proteins and nucleic acids from whole human blood in order to potentially improve detection and diagnosis.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jun 21, 2021
- Source ID
- 10.1186/s12951-021-00859-8
Entities
People
- Alexandra N. Ii
- Benjamin Lepene
- Kylene Kehn-Hall
- Monique van Hoek
- Shih-Chao Lin
- Weidong Zhou
Organizations
- Defense Threat Reduction Agency