Tracking macrophages in diabetic neuropathy with two-color nanoemulsions for near-infrared fluorescent imaging and microscopy
Abstract
The incidence of diabetes and diabetic peripheral neuropathy continues to rise, and studies have shown that macrophages play an important role in their pathogenesis. To date, macrophage tracking has largely been achieved using genetically-encoded fluorescent proteins. Here we present a novel two-color fluorescently labeled perfluorocarbon nanoemulsion (PFC-NE) designed to monitor phagocytic macrophages in diabetic neuropathy in vitro and in vivo using non-invasive near-infrared fluorescent (NIRF) imaging and fluorescence microscopy.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Dec 23, 2021
- Source ID
- 10.1186/s12974-021-02365-y
Entities
People
- Andrew J Shepherd
- Caitlin V. Crelli
- Hoang Vu Pham
- James M. Nichols
- Jelena M. Janjic
- Lu Liu
Organizations
- United States Department of Defense