Orthogonal control of gene expression in plants using synthetic promoters and CRISPR-based transcription factors
Abstract
The construction and application of synthetic genetic circuits is frequently improved if gene expression can be orthogonally controlled, relative to the host. In plants, orthogonality can be achieved via the use of CRISPR-based transcription factors that are programmed to act on natural or synthetic promoters. The construction of complex gene circuits can require multiple, orthogonal regulatory interactions, and this in turn requires that the full programmability of CRISPR elements be adapted to non-natural and non-standard promoters that have few constraints on their design. Therefore, we have developed synthetic promoter elements in which regions upstream of the minimal 35S CaMV promoter are designed from scratch to interact via programmed gRNAs with dCas9 fusions that allow activation of gene expression.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Mar 29, 2022
- Source ID
- 10.1186/s13007-022-00867-1
Entities
People
- Andrew D Ellington
- Elizabeth C. Gardner
- Jimmy D. Gollihar
- Junghyun Kim
- Nestor Rodriguez
- Shaunak Kar
- Sibum Sung
- Yogendra Bordiya
Organizations
- Defense Advanced Research Projects Agency