Dipeptide repeat proteins inhibit homology-directed DNA double strand break repair in C9ORF72 ALS/FTD

Abstract

The C9ORF72 hexanucleotide repeat expansion is the most common known genetic cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), two fatal age-related neurodegenerative diseases. The C9ORF72 expansion encodes five dipeptide repeat proteins (DPRs) that are produced through a non-canonical translation mechanism. Among the DPRs, proline-arginine (PR), glycine-arginine (GR), and glycine-alanine (GA) are the most neurotoxic and increase the frequency of DNA double strand breaks (DSBs). While the accumulation of these genotoxic lesions is increasingly recognized as a feature of disease, the mechanism(s) of DPR-mediated DNA damage are ill-defined and the effect of DPRs on the efficiency of each DNA DSB repair pathways has not been previously evaluated.

Document Details

Document Type
Pub Defense Publication
Publication Date
Feb 24, 2020
Source ID
10.1186/s13024-020-00365-9

Entities

People

  • Abbas Abdallah
  • Christian Mueller
  • Claes Wahlestedt
  • Gabriel Gaidosh
  • Mathew J. Rybin
  • Melina Ramic
  • Michael Benatar
  • Nadja S. Andrade
  • Nancy T. Chee
  • Rustam Esanov
  • Sadhana Anatha
  • Samuel Del’olio
  • Tania F. Gendron
  • Tyler C. Huff
  • Wenjun Liu
  • Yanbin Zhang
  • Zane Zeier

Organizations

  • National Institute of Neurological Disorders and Stroke
  • National Institutes of Health
  • United States Department of Defense

Tags

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Traumatic Brain Injury (TBI) and Cognitive Aging in the Guam and Border Populations Affected by Alzheimer's Disease and Tau-Associated Dementias.

Technology Areas

  • Biotechnology