Sustained postconfluent culture of human mammary epithelial cells enriches for luminal and c-Kit+ subtypes
Abstract
A challenge in human mammary epithelial cell (HMEC) culture is sustaining the representation of competing luminal, myoepithelial, and progenitor lineages over time. As cells replicate in culture, myoepithelial cells come to dominate the composition of the culture with serial passaging. This drift in composition presents a challenge for studying luminal and progenitor cells, which are prospective cells of origin for most breast cancer subtypes.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 18, 2023
- Source ID
- 10.1186/s13058-022-01595-z
Entities
People
- Eric G. Carlson
- Mark A LaBarge
- Masaru Miyano
- Michael E. Todhunter
- Stefan Hinz
Organizations
- American Cancer Society
- United States Department of Defense