THF co-solvent pretreatment prevents lignin redeposition from interfering with enzymes yielding prolonged cellulase activity
Abstract
Conventional aqueous dilute sulfuric acid (DSA) pretreatment of lignocellulosic biomass facilitates hemicellulose solubilization and can improve subsequent enzymatic digestibility of cellulose to fermentable glucose. However, much of the lignin after DSA pretreatment either remains intact within the cell wall or readily redeposits back onto the biomass surface. This redeposited lignin has been shown to reduce enzyme activity and contribute to rapid enzyme deactivation, thus, necessitating significantly higher enzyme loadings than deemed economical for biofuel production from biomass.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Mar 09, 2021
- Source ID
- 10.1186/s13068-021-01904-2
Entities
People
- Abhishek S. Patri
- Arthur J. Ragauskas
- Chang G. Yoo
- Charles E Wyman
- Charles M. Cai
- David J Kisailus
- Rajeev Kumar
- Ramya Mohan
- Yunqiao Pu
Organizations
- Office of Biological and Environmental Research