Characterization of human norovirus binding to gut-associated bacterial ligands
Abstract
Research suggests human norovirus binding to histo-blood group antigen (HBGA)-like molecules on enteric bacteria may enhance viral pathogenesis; however, the properties of these bacterial ligands are not well known. Previous work identified, but did not characterize, seven norovirus-binding bacteria. To further examine this bacteria–virus binding interaction, enteric bacteria were analyzed via Western blot with anti-HBGA antibodies and lectins targeting HBGA-associated sugar components. Virus overlay assays using capsids from six different human norovirus strains further identified responsible ligands and strain dependent binding properties.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Sep 23, 2019
- Source ID
- 10.1186/s13104-019-4669-2
Entities
People
- Erin A Almand
- Lee-ann Jaykus
- Matthew D. Moore
Organizations
- Air Force Institute of Technology
- National Institute of Food and Agriculture