Panspecies molecular assays detect viral pathogens missed by real-time PCR/reverse-transcriptase PCR among pneumonia patients, Sarawak, Malaysia
Abstract
In a year-long pneumonia etiology study conducted June 2017 to May 2018 in Sarawak, Malaysia, 599 patients’ nasopharyngeal swab specimens were studied with real-time polymerase chain reaction (rPCR)/ reverse-transcription (rRT-PCR) assays for respiratory pathogens known to contribute to the high burden of lower respiratory tract infections. The study team sought to compare real-time assay results with panspecies conventional molecular diagnostics to compare sensitivities and learn if novel viruses had been missed.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 12, 2020
- Source ID
- 10.1186/s40794-020-00114-2
Entities
People
- Antoinette Berita
- Diego Galan
- Emily S. Bailey
- Gregory C Gray
- Jakie Ting
- Jane K. Fieldhouse
- John A. Lednicky
- Kerry A. Mallinson
- King-ching Hii
- Patrick J. Blair
- See-chang Wong
- Son T. Than
- Teck-hock Toh
- Tham Thi Nguyen
- Toh-mee Wong