Optimal induction of antigen-specific CD8+ T cell responses requires bystander cell participation
Abstract
Efficient activation of specific immune responses requires a concerted interaction between T cells and antigen-presenting cells. A requirement for bystander participation of CD4+ T cells for expansion and maintenance of memory CD8+ T cells has been noted in several models, but a role with regard to effector CD8+ T responses has not been well-defined. In this report, the requirement of bystander participation for optimal induction of antigen-specific CD8+ T cell effector function was determined by directly quantitating antigen-specific interferon-γ (IFN-γ) CD8+ T cell responses by enzyme-linked immunospot assays, and by indirectly evaluating induction of the chemokine monokine induced by IFN-γ as a marker for IFN-γ-mediated effector function. Our results demonstrate that bystander cell participation, mediated by CD4+ T cell and natural killer (NK) cells, is required for optimal induction of antigen-specific CD8+ T cell effector responses. Our data further establish a novel role for NK cells in the activation of antigen-specific immune responses.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Dec 01, 2002
- Source ID
- 10.1189/jlb.72.6.1164
Entities
People
- Daniel J. Carucci
- Denise L. Doolan
- Gary T. Brice
- Norma L Graber
Organizations
- Henry M. Jackson Foundation for the Advancement of Military Medicine
- Johns Hopkins University
- Naval Medical Research Center