Mitochondrial dynamics and respiration within cells with increased open pore cytoskeletal meshes
Abstract
The cytoskeletal architecture directly affects the morphology, motility, and tensional homeostasis of the cell. In addition, the cytoskeleton is important for mitosis, intracellular traffic, organelle motility, and even cellular respiration. The organelle responsible for a majority of the energy conversion for the cell, the mitochondrion, has a dependence on the cytoskeleton for mobility and function. In previous studies, we established that cytoskeletal inhibitors altered the movement of the mitochondria, their morphology, and their respiration in human dermal fibroblasts. Here, we use this protocol to investigate applicability of power law diffusion to describe mitochondrial locomotion, assessment of rates of fission and fusion in healthy and diseased cells, and differences in mitochondria locomotion in more open networks either in response to cytoskeletal destabilizers or by cell line.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 01, 2017
- Source ID
- 10.1242/bio.029009
Entities
People
- David H. Jang
- David M Eckmann
- Frances C. Shofer
- Martha E. Grady
- Sarah C. Seeger
Organizations
- National Heart, Lung, and Blood Institute
- Office of Naval Research
- University of Kentucky
- University of Pennsylvania