Detection of changes in mitochondrial hydrogen sulfide i n vivo in the fish model Poecilia mexicana (Poeciliidae)
Abstract
In this paper, we outline the use of a mitochondria-targeted ratiometric mass spectrometry probe, MitoA, to detect in vivo changes in mitochondrial hydrogen sulfide (H2S) in Poecilia mexicana (family Poeciliidae). MitoA is introduced via intraperitoneal injection into the animal and is taken up by mitochondria, where it reacts with H2S to form the product MitoN. The MitoN/MitoA ratio can be used to assess relative changes in the amounts of mitochondrial H2S produced over time. We describe the use of MitoA in the fish species P. mexicana to illustrate the steps for adopting the use of MitoA in a new organism, including extraction and purification of MitoA and MitoN from tissues followed by tandem mass spectrometry. In this proof-of-concept study we exposed H2S tolerant P. mexicana to 59 µM free H2S for 5 h, which resulted in increased MitoN/MitoA in brain and gills, but not in liver or muscle, demonstrating increased mitochondrial H2S levels in select tissues following whole-animal H2S exposure. This is the first time that accumulation of H2S has been observed in vivo during whole-animal exposure to free H2S using MitoA.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- May 09, 2019
- Source ID
- 10.1242/bio.041467
Entities
People
- Gigi Y Lau
- Jeffrey G. Richards
- Michael P. Murphy
- Michael Tobler
- Nicholas Barts
- Richard C. Hartley
- Sabine Arndt
Organizations
- Army Research Office
- Johannes Gutenberg University Mainz
- Kansas State University
- Medical Research Council Canada
- National Science Foundation
- Natural Sciences and Engineering Research Council
- Office of Naval Research
- University of British Columbia
- University of Cambridge
- University of Glasgow
- Wellcome