Hybridization chain reaction enables a unified approach to multiplexed, quantitative, high-resolution immunohistochemistry and in situ hybridization
Abstract
RNA in situ hybridization based on the mechanism of the hybridization chain reaction (HCR) enables multiplexed, quantitative, high-resolution RNA imaging in highly autofluorescent samples, including whole-mount vertebrate embryos, thick brain slices and formalin-fixed paraffin-embedded tissue sections. Here, we extend the benefits of one-step, multiplexed, quantitative, isothermal, enzyme-free HCR signal amplification to immunohistochemistry, enabling accurate and precise protein relative quantitation with subcellular resolution in an anatomical context. Moreover, we provide a unified framework for simultaneous quantitative protein and RNA imaging with one-step HCR signal amplification performed for all target proteins and RNAs simultaneously.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Nov 15, 2021
- Source ID
- 10.1242/dev.199847
Entities
People
- Harry Choi
- Maayan Schwarzkopf
- Mike C. Liu
- N. Husain
- Niles A. Pierce
- Rachel Ives
- Samuel J. Schulte
Organizations
- California Institute of Technology
- Defense Advanced Research Projects Agency
- National Institutes of Health