Dynein-mediated microtubule translocation powering neurite outgrowth in chick and Aplysia neurons requires microtubule assembly
Abstract
Previously, we have shown that bulk microtubule (MT) movement correlates with neurite elongation, and blocking either dynein activity or MT assembly inhibits both processes. However, whether the contributions of MT dynamics and dynein activity to neurite elongation are separate or interdependent is unclear. Here, we investigated the underlying mechanism by testing the roles of dynein and MT assembly in neurite elongation of Aplysia and chick neurites using time-lapse imaging, fluorescent speckle microscopy, super-resolution imaging and biophysical analysis. Pharmacologically inhibiting either dynein activity or MT assembly reduced neurite elongation rates as well as bulk and individual MT anterograde translocation. Simultaneously suppressing both processes did not have additive effects, suggesting a shared mechanism of action. Single-molecule switching nanoscopy revealed that inhibition of MT assembly decreased the association of dynein with MTs. Finally, inhibiting MT assembly prevented the rise in tension induced by dynein inhibition. Taken together, our results suggest that MT assembly is required for dynein-driven MT translocation and neurite outgrowth.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 15, 2020
- Source ID
- 10.1242/jcs.232983
Entities
People
- Daniel Suter
- Donghan Ma
- Fang Huang
- Jessica E. Stone
- Kristi Mcelmurry
- Kyle E Miller
- Lucas Fix
- Michelle Steidemann
- Phillip Lamoureux
- Yueyun Zhang
Organizations
- Defense Advanced Research Projects Agency
- Michigan State University
- National Institutes of Health
- National Science Foundation
- Purdue University