Efficient photobleaching of rhodamine 6G by a single UV pulse
Abstract
While photobleaching can be detrimental in applications focusing on fluorescence as it lowers the signal strength, it can advantageously provide non-fluorescent tracers in a fluorescent flow and hence be implemented in tracking techniques such as molecular tagging velocimetry (MTV). The photobleaching of rhodamine 6G under a single UV pulse is described with a simple three-level model of fluorescence. It offers a convenient estimate of the order of magnitude of irradiance required to observe significant photobleaching for a uniform beam. In an effort to improve the application to MTV, analytical formulas enable to determine the photobleached signal strength from a Gaussian UV laser beam overlapping with a green laser sheet and imaged from the side, as well as the apparent width of the imaged photobleached line.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jul 21, 2021
- Source ID
- 10.1364/ao.431209
Entities
People
- Charles Fort
- Philippe M. Bardet
Organizations
- George Washington University
- Office of Naval Research