Multi-resolution open-top light-sheet microscopy to enable efficient 3D pathology workflows
Abstract
Open-top light-sheet (OTLS) microscopes have been developed for user-friendly and versatile high-throughput 3D microscopy of thick specimens. As with all imaging modalities, spatial resolution trades off with imaging and analysis times. A hierarchical multi-scale imaging workflow would therefore be of value for many volumetric microscopy applications. We describe a compact multi-resolution OTLS microscope, enabled by a novel solid immersion meniscus lens (SIMlens), which allows users to rapidly transition between air-based objectives for low- and high-resolution 3D imaging. We demonstrate the utility of this system by showcasing an efficient 3D analysis workflow for a diagnostic pathology application.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Oct 22, 2020
- Source ID
- 10.1364/boe.408684
Entities
People
- Adam K Glaser
- Hongyi Huang
- Jonathan T C Liu
- Lawrence D. True
- Lindsey A. Barner
Organizations
- National Cancer Institute
- National Institutes of Health
- National Science Foundation
- United States Department of Defense