Rapid and precise tracking of water influx and efflux across cell membranes induced by a pulsed electric field
Abstract
Quantitative measurements of water content within a single cell are notoriously difficult. In this work, we introduce a single-shot optical method for tracking the intracellular water content, by mass and volume, of a single cell at video rate. We utilize quantitative phase imaging and a priori knowledge of a spherical cellular geometry, leveraging a two-component mixture model to compute the intracellular water content. We apply this technique to study CHO-K1 cells responding to a pulsed electric field, which induces membrane permeabilization and rapid water influx or efflux depending upon the osmotic environment. The effects of mercury and gadolinium on water uptake in Jurkat cells following electropermeabilization are also examined.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 04, 2023
- Source ID
- 10.1364/boe.485627
Entities
People
- Allen S. Kiester
- Anna Sedelnikova
- Bennett L. Ibey
- Jennifer Tran
- Joel N. Bixler
- Sean P O'Connor
- Stacey L Martens
- Zachary A Steelman
- Zachary N. Coker
Organizations
- Air Force Office of Scientific Research
- Air Force Research Laboratory
- United States Air Force
- United States Department of Energy
- University of Wisconsin–Madison