Two-dimensional random access multiphoton spatial frequency modulated imaging
Abstract
Spatial frequency modulated imaging (SPIFI) enables the use of an extended excitation source for linear and nonlinear imaging with single element detection. To date, SPIFI has only been used with fixed excitation source geometries. Here, we explore the potential for the SPIFI method when a spatial light modulator (SLM) is used to program the excitation source, opening the door to a more versatile, random access imaging environment. In addition, an in-line, quantitative pulse compensation and measurement scheme is demonstrated using a new technique, spectral phase and amplitude retrieval and compensation (SPARC). This enables full characterization of the light exposure conditions at the focal plane of the random access imaging system, an important metric for optimizing, and reporting imaging conditions within specimens.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 02, 2020
- Source ID
- 10.1364/oe.378460
Entities
People
- Alyssa M. Allende Motz
- Charles Durfee
- Christopher L. Hoy
- Daniel E Adams
- Jeff Squier
- Jeffrey J. Field
- John Czerski
- Randy A. Bartels
Organizations
- Air Force Office of Scientific Research
- National Institutes of Health
- National Science Foundation