Spinning pupil aberration measurement for anisoplanatic deconvolution

Abstract

The aberrations in an optical microscope are commonly measured and corrected at one location in the field of view, within the so-called isoplanatic patch. Full-field correction is desirable for high-resolution imaging of large specimens. Here we present, to the best of our knowledge, a novel wavefront detector, based on pupil sampling with subapertures, measuring the aberrated wavefront phase at each position of the specimen. Based on this measurement, we propose a region-wise deconvolution that provides an anisoplanatic reconstruction of the sample image. Our results indicate that the measurement and correction of the aberrations can be performed in a wide-field fluorescence microscope over its entire field of view.

Document Details

Document Type
Pub Defense Publication
Publication Date
Jun 14, 2021
Source ID
10.1364/ol.427518

Entities

People

  • Andrea Bassi
  • Daniele Ancora
  • Stefano Bonora
  • Tommaso Furieri

Organizations

  • Air Force Research Laboratory
  • Consiglio Nazionale delle Ricerche
  • Marie SkÅ‚odowska-Curie Actions
  • Office of Naval Research Global
  • Polytechnic University of Milan
  • University of Padua

Tags

Fields of Study

  • Physics

Readers

  • Image Processing and Computer Vision.