Cell-specific three-photon-fluorescence brain imaging: neurons, astrocytes, and gliovascular interfaces
Abstract
We present brain imaging experiments on rat cortical areas, demonstrating that, when combined with a suitable high-brightness, cell-specific genetically encoded fluorescent marker, three-photon-excited fluorescence (3PEF), enables subcellular-resolution, cell-specific 3D brain imaging that is fully compatible and readily integrable with other nonlinear-optical imaging modalities, including two-photon-fluorescence and harmonic-generation microscopy. With laser excitation provided by sub-100-fs, 1.25-µm laser pulses, cell-specific 3PEF from astrocytes and their processes detected in parallel with a three-photon-resonance-enhanced third harmonic from blood vessels is shown to enable a high-contrast 3D imaging of gliovascular interfaces.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Feb 06, 2020
- Source ID
- 10.1364/ol.45.000836
Entities
People
- A. A. Ivanov
- A. B. Fedotov
- A. S. Chebotarev
- Aleksandr Lanin
- Aleksey Zheltikov
- D. A. Kotova
- D. S. Bilan
- Ilya V Kelmanson
- M. S. Pochechuev
- V. S. Tarabykin
- V. V. Belousov
Organizations
- Office of Naval Research
- Robert A. Welch Foundation
- Russian Center for Science Information
- Russian Science Foundation