Circumventing the optical diffraction limit with customized speckles
Abstract
Speckle patterns have been used widely in imaging techniques such as ghost imaging, dynamic speckle illumination microscopy, structured illumination microscopy, and photoacoustic fluctuation imaging. Recent advances in the ability to control the statistical properties of speckles has enabled the customization of speckle patterns for specific imaging applications. In this work, we design and create special speckle patterns for parallelized nonlinear pattern-illumination microscopy based on fluorescence photoswitching. We present a proof-of-principle experimental demonstration where we obtain a spatial resolution three times higher than the diffraction limit of the illumination optics in our setup. Furthermore, we show that tailored speckles vastly outperform standard speckles. Our work establishes that customized speckles are a potent tool in parallelized super-resolution microscopy.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jan 22, 2021
- Source ID
- 10.1364/optica.411007
Entities
People
- Hasan Yılmaz
- Hui Cao
- Joerg Bewersdorf
- Mengyuan Sun
- Nicholas Bender
Organizations
- National Institute of General Medical Sciences
- National Institutes of Health
- National Science Foundation
- Office of Naval Research
- Wellcome