Flexible method for generating needle-shaped beams and its application in optical coherence tomography
Abstract
Needle-shaped beams (NBs) featuring a long depth-of-focus (DOF) can drastically improve the resolution of microscopy systems. However, thus far, the implementation of a specific NB has been onerous due to the lack of a common, flexible generation method. Here we develop a spatially multiplexed phase pattern that creates many axially closely spaced foci as a universal platform for customizing various NBs, allowing flexible manipulations of beam length and diameter, uniform axial intensity, and sub-diffraction-limit beams. NBs designed via this method successfully extended the DOF of our optical coherence tomography (OCT) system. It revealed clear individual epidermal cells of the entire human epidermis, fine structures of human dermal-epidermal junction in a large depth range, and a high-resolution dynamic heartbeat of alive Drosophila larvae.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jul 22, 2022
- Source ID
- 10.1364/optica.456894
Entities
People
- Adam De La Zerda
- Aidan Van Vleck
- Cheng Huang
- Jingjing Zhao
- Kavita Y Sarin
- Kenzo Ichimura
- Lin Du
- Sumaira Z. Aasi
- Yonatan Winetraub
Organizations
- Alexander & Margaret Stewart Trust
- Chan Zuckerberg Initiative
- Damon Runyon Cancer Research Foundation
- Donald E. and Delia B. Baxter Foundation
- Mary Kay Foundation
- National Institutes of Health
- Stanford University
- Stanford University School of Medicine
- United States Air Force
- University of Pennsylvania