Fluorescent nanodiamonds for characterization of nonlinear microscopy systems
Abstract
Characterizing the performance of fluorescence microscopy and nonlinear imaging systems is an essential step required for imaging system optimization and quality control during longitudinal experiments. Emerging multimodal nonlinear imaging techniques require a new generation of microscopy calibration targets that are not susceptible to bleaching and can provide a contrast across the multiple modalities. Here, we present a nanodiamond-based calibration target for microscopy, designed for facilitating reproducible measurements at the object plane. The target is designed to support day-to-day instrumentation development efforts in microscopy laboratories. The images of a phantom contain information about the imaging performance of a microscopy system across multiple spectral windows and modalities. Since fluorescent nanodiamonds are not prone to bleaching, the proposed imaging target can serve as a standard, shelf-stable sample to provide rapid reference measurements for ensuring consistent performance of microscopy systems in microscopy laboratories and imaging facilities.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Nov 03, 2021
- Source ID
- 10.1364/prj.434236
Entities
People
- Aneesh Alex
- Jaena Park
- Mantas Zurauskas
- Stephen A. Boppart
- Steve R. Hood
Organizations
- Air Force Office of Scientific Research
- GSK
- National Institutes of Health
- University of Illinois Urbana–Champaign