Imd pathway-specific immune assays reveal NF-κB stimulation by viral RNA PAMPs in Aedes aegypti Aag2 cells

Abstract

The mosquito Aedes aegypti is a major vector for the arthropod-borne viruses (arboviruses) chikungunya, dengue, yellow fever and Zika viruses. Vector immune responses pose a major barrier to arboviral transmission, and transgenic insects with altered immunity have been proposed as tools for reducing the global public health impact of arboviral diseases. However, a better understanding of virus-immune interactions is needed to progress the development of such transgenic insects. Although the NF-κB-regulated Toll and ‘immunodeficiency’ (Imd) pathways are increasingly thought to be antiviral, relevant pattern recognition receptors (PRRs) and pathogen-associated molecular patterns (PAMPs) remain poorly characterised in A. aegypti.

Document Details

Document Type
Pub Defense Publication
Publication Date
Feb 16, 2021
Source ID
10.1371/journal.pntd.0008524

Entities

People

  • Ana Fernández-Sesma
  • Andalus Ayaz
  • Andrew D Davidson
  • Kevin Maringer
  • Tiffany A Russell

Organizations

  • Defense Advanced Research Projects Agency
  • Icahn School of Medicine at Mount Sinai
  • Medical Research Council
  • National Institute of Allergy and Infectious Diseases
  • Wellcome Trust

Tags

Fields of Study

  • Biology

Readers

  • Immunology
  • Infectious Disease/Epidemiology
  • Vector-Borne Disease and Entomology

Technology Areas

  • AI & ML