Automatic reagent handling and assay processing of human biospecimens inside a transportation container for a medical disaster response against radiation

Abstract

Biological materials can be shipped off-site for diagnostic, therapeutic and research purposes. They usually are kept in certain environments for their final application during transportation. However, active reagent handling during transportation from a collection site to a laboratory or biorepository has not been reported yet. In this paper, we show the application of a micro-controlled centrifugal microfluidic system inside a shipping container that can add reagent to an actively cultured human blood sample during transportation to ensure a rapid biodosimetry of cytokinesis-block micronucleus (CBMN) assay. The newly demonstrated concept could have a significant impact on rapid biodosimetry triage for medical countermeasure in a radiological disaster. It also opens a new capability in accelerated sample processing during transportation for biomedical and healthcare applications.

Document Details

Document Type
Pub Defense Publication
Publication Date
May 20, 2022
Source ID
10.1371/journal.pone.0268508

Entities

People

  • Adam R. Akkad
  • Adarsh Ramakumar
  • Alan Norquist
  • Brett Duane
  • David J. Brenner
  • Frederic Zenhausern
  • Jian Gu

Organizations

  • Armed Forces Radiobiology Research Institute
  • Congressionally Directed Medical Research Programs

Tags

Readers

  • Computer Science/Computer Engineering/Data Science/Digital Signal Processing.
  • Maritime Security/Maritime Homeland Security
  • Toxicology/Environmental Toxicology

Technology Areas

  • Biotechnology