Soluble chitosan derivative treats wound infections and promotes wound healing in a novel MRSA-infected porcine partial-thickness burn wound model
Abstract
Burns are physically debilitating and potentially fatal injuries. The most common etiology of burn wound infections in the US is methicillin-resistantStaphylococcus aureus(MRSA), which is particularly recalcitrant when biofilms form. The current standard of care, silver sulfadiazine (SSD) is effective in reducing bacterial load, but less effective in improving burn wound healing. New treatments that can manage infection while simultaneously improving healing would provide a benefit in the treatment of burns. Porcine models are frequently used as a model for human wound healing but can be expensive due to the need to separate wounds to avoid cross contamination. The porcine model developed in this study offers the capability to study multiple partial thickness burn wound (PTBW) sites on a single animal with minimal crosstalk to study wound healing, infection, and inflammation. The current study evaluates a wound rinse and a wound gel formulated with a non-toxic, polycationic chitosan derivative that is hypothesized to manage infection while also promoting healing, providing a potential alternate to SSD. Studiesin vitroand in this PTBW porcine model compare treatment with the chitosan derivative formulations to SSD. The wound rinse and wound gel are observed to disrupt mature MRSA biofilmsin vitroand reduce the MRSA loadin vivo when compared to that of the standard of care.In vivodata further show increased re-epithelialization and faster healing in burns treated with wound rinse/gel as compared to SSD. Taken together, the data demonstrate the potential of the wound rinse/gel to significantly enhance healing, promote re-epithelialization, and reduce bacterial burden in infected PTBW using an economical porcine model.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Oct 14, 2022
- Source ID
- 10.1371/journal.pone.0274455
Entities
People
- Alex Repko
- Ali Ayyash
- Allister Loughran
- Asim Ejaz
- Deokyeol Kim
- Francesco M Egro
- Jenny Ziembicki
- Kacey G. Marra
- M Asher Schusterman
- P. Rubin
- Shenda M. Baker
- Stacy M. Towsend
- Vidya P Narayanaswamy
Organizations
- Armed Forces Institute of Regenerative Medicine