Tentacle Probes™: Differentiation of Difficult Single-Nucleotide Polymorphisms and Deletions by Presence or Absence of a Signal in Real-Time PCR
Abstract
Background: False-positive results are a common problem in real-time PCR identification of DNA sequences that differ from near neighbors by a single-nucleotide polymorphism (SNP) or deletion. Because of a lack of sufficient probe specificity, post-PCR analysis, such as a melting curve, is often required for mutation differentiation.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Dec 01, 2007
- Source ID
- 10.1373/clinchem.2007.091488
Entities
People
- Brent C Satterfield
- David A. Kulesh
- David A. Norwood
- Jay Aa West
- Leonard P. Wasieloski Jr.
- Michael R Caplan
Organizations
- Arizona State University
- Defense Threat Reduction Agency
- National Institute of Dental and Craniofacial Research
- National Institutes of Health
- Oak Ridge Institute for Science and Education
- United States Army Medical Research Institute of Infectious Diseases
- United States Department of Energy
- United States Department of Homeland Security