Genetic Modifiers ofdFMR1Encode RNA Granule Components in Drosophila
Abstract
Mechanisms of neuronal mRNA localization and translation are of considerable biological interest. Spatially regulated mRNA translation contributes to cell-fate decisions and axon guidance during development, as well as to long-term synaptic plasticity in adulthood. The Fragile-X Mental Retardation protein (FMRP/dFMR1) is one of the best-studied neuronal translational control molecules and here we describe the identification and early characterization of proteins likely to function in the dFMR1 pathway. Induction of the dFMR1 in sevenless-expressing cells of the Drosophila eye causes a disorganized (rough) eye through a mechanism that requires residues necessary for dFMR1/FMRP's translational repressor function. Several mutations in dco, orb2, pAbp, rm62, and smD3 genes dominantly suppress the sev-dfmr1 rough-eye phenotype, suggesting that they are required for dFMR1-mediated processes. The encoded proteins localize to dFMR1-containing neuronal mRNPs in neurites of cultured neurons, and/or have an effect on dendritic branching predicted for bona fide neuronal translational repressors. Genetic mosaic analyses indicate that dco, orb2, rm62, smD3, and dfmr1 are dispensable for translational repression of hid, a microRNA target gene, known to be repressed in wing discs by the bantam miRNA. Thus, the encoded proteins may function as miRNA- and/or mRNA-specific translational regulators in vivo.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 01, 2009
- Source ID
- 10.1534/genetics.109.103234
Entities
People
- Anne-marie J Cziko
- Cathal T Mccann
- Daniela C Zarnescu
- Iris C Howlett
- Konrad E Zinsmaier
- Mani Ramaswami
- Rebecca P Duncan
- Rene Luedemann
- Roy R Parker
- Scott A. Barbee
Organizations
- Australian RL Commission
- Trinity College Dublin
- University of Arizona