Mass spectrometric analysis of bioactive conditioned media of bacteria isolated from reptilian gut
Abstract
Aim: To determine whether selected gut bacteria of crocodile exhibit antibacterial properties. Materials & methods: Two bacteria isolated from Crocodylus porosus gut were used, namely: Pseudomonas aeruginosa and Aeromonas dhakensis. Conditioned media were tested against pathogenic bacteria and metabolites were analyzed using liquid chromatography-mass spectrometry. Results & conclusion: Antibacterial assays revealed that conditioned media showed potent effects against pathogenic Gram-positive and Gram-negative bacteria. LC–MS revealed identity of 210 metabolites. The abundant metabolites were, N-Acetyl-L-tyrosine, Acetaminophen, Trans-Ferulic acid, N, N-Dimethylformamide, Pyrocatechol, Cyclohexanone, Diphenhydramine, Melatonin, Gamma–terpinene, Cysteamine, 3-phenoxypropionic acid, Indole-3-carbinol, Benzaldehyde, Benzocaine, 2-Aminobenzoic acid, 3-Methylindole. These findings suggest that crocodile gut bacteria are potential source of novel bioactive molecules that can be utilized as pre/post/antibiotics for the benefit of human health.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jun 01, 2023
- Source ID
- 10.2144/fsoa-2023-0030
Entities
People
- Hamza Mohammad Al-hroub
- Mohammad Harb Semreen
- Naveed Ahmed Khan
- Nelson Cruz Soares
- Noor Akbar
- Ruqaiyyah Siddiqui
- Sutherland K Maciver
Organizations
- Air Force Office of Scientific Research
- American University of Sharjah
- University of Edinburgh
- University of Sharjah
- İstinye University