Biotechnological Applications of Protein Splicing
Abstract
Protein splicing domains, also called inteins, have become a powerful biotechnological tool for applications involving molecular biology and protein engineering. Early applications of inteins focused on self-cleaving affinity tags, generation of recombinant polypeptide α-thioesters for the production of semisynthetic proteins and backbone cyclized polypeptides. The discovery of naturallyoccurring split-inteins has allowed the development of novel approaches for the selective modification of proteins both in vitro and in vivo. This review gives a general introduction to protein splicing with a focus on their role in expanding the applications of intein-based technologies in protein engineering and chemical biology.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Mar 27, 2019
- Source ID
- 10.2174/1389203720666190208110416
Entities
People
- Corina Sarmiento
- Julio A Camarero
Organizations
- Congressionally Directed Medical Research Programs
- Crohn's & Colitis Foundation of America
- National Institutes of Health
- University of Southern California