STAT1 Dissociates Adipose Tissue Inflammation From Insulin Sensitivity in Obesity

Abstract

Obesity fosters low-grade inflammation in white adipose tissue (WAT) that may contribute to the insulin resistance that characterizes type 2 diabetes. However, the causal relationship of these events remains unclear. The established dominance of STAT1 function in the immune response suggests an obligate link between inflammation and the comorbidities of obesity. To this end, we sought to determine how STAT1 activity in white adipocytes affects insulin sensitivity. STAT1 expression in WAT inversely correlated with fasting plasma glucose in both obese mice and humans. Metabolomic and gene expression profiling established STAT1 deletion in adipocytes (STAT1a-KO) enhanced mitochondrial function and accelerated tricarboxylic acid cycle flux coupled with reduced fat cell size in subcutaneous WAT depots. STAT1a-KO reduced WAT inflammation, but insulin resistance persisted in obese mice. Rather, elimination of type I cytokine interferon-γ activity enhanced insulin sensitivity in diet-induced obesity. Our findings reveal a permissive mechanism that bridges WAT inflammation to whole-body insulin sensitivity.

Document Details

Document Type
Pub Defense Publication
Publication Date
Sep 29, 2020
Source ID
10.2337/db20-0384

Entities

People

  • Aaron R Cox
  • Cristian Coarfa
  • David A. Bader
  • Dennis T. Villareal
  • Huaizhu Wu
  • Jessica B. Felix
  • Kang Ho Kim
  • Kimal Rajapakshe
  • Nagireddy Putluri
  • Natasha Chernis
  • Peter M. Masschelin
  • Pradip K. Saha
  • Reina Armamento-villareal
  • Robert Sharp
  • Sean M Hartig
  • Vasanta Putluri
  • Zeqin Lian

Organizations

  • American Diabetes Association
  • Baylor College of Medicine
  • Cancer Prevention and Research Institute of Texas
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development
  • Michael E. DeBakey Veterans Affairs Medical Center in Houston
  • National Cancer Institute
  • National Institute of Diabetes and Digestive and Kidney Diseases
  • United States Department of Defense
  • United States Department of Veterans Affairs

Tags

Fields of Study

  • Medicine

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