Whole-cell vaccine candidates induce a protective response against virulent Acinetobacter baumannii

Abstract

Acinetobacter baumannii causes multi-system diseases in both nosocomial settings and a pre-disposed general population. The bacterium is not only desiccation-resistant but also notoriously resistant to multiple antibiotics and drugs of last resort including carbapenem, colistin, and sulbactam. The World Health Organization has categorized carbapenem-resistant A. baumannii at the top of its critical pathogen list in a bid to direct urgent countermeasure development. Several early-stage vaccines have shown a range of efficacies in healthy mice, but no vaccine candidates have advanced into clinical trials. Herein, we report our findings that both an ionizing γ-radiation-inactivated and a non-ionizing ultraviolet C-inactivated whole-cell vaccine candidate protects neutropenic mice from pulmonary challenge with virulent AB5075, a particularly pathogenic isolate. In addition, we demonstrate that a humoral response is sufficient for this protection via the passive immunization of neutropenic mice.

Document Details

Document Type
Pub Defense Publication
Publication Date
Sep 27, 2022
Source ID
10.3389/fimmu.2022.941010

Entities

People

  • Christine M. Czintos
  • Daniel V Zurawski
  • David A. Macleod
  • Gregory J. Tobin
  • Heather N. Meeks
  • John K. Tobin
  • Mariel G. Escatte
  • Michael J. Daly
  • Naomi J. P. E. R. Tasker
  • Rania Abu-taleb
  • Ruth V. Bushnell
  • Stephen J Dollery
  • Taralyn J. Wiggins
  • Wanwen Su
  • Yonas A. Alamneh

Organizations

  • Defense Threat Reduction Agency

Tags

Fields of Study

  • Biology

Readers

  • Immunology
  • Military/Explosive Ordnance Disposal (EOD) Technology
  • Oncology

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech