Direct Interaction of Avian Cryptochrome 4 with a Cone Specific G-Protein
Abstract
Background: Night-migratory birds sense the Earth’s magnetic field by an unknown molecular mechanism. Theoretical and experimental evidence support the hypothesis that the light-induced formation of a radical-pair in European robin cryptochrome 4a (ErCry4a) is the primary signaling step in the retina of the bird. In the present work, we investigated a possible route of cryptochrome signaling involving the α-subunit of the cone-secific heterotrimeric G protein from European robin. Methods: Protein–protein interaction studies include surface plasmon resonance, pulldown affinity binding and Förster resonance energy transfer. Results: Surface plasmon resonance studies showed direct interaction, revealing high to moderate affinity for binding of non-myristoylated and myristoylated G protein to ErCry4a, respectively. Pulldown affinity experiments confirmed this complex formation in solution. We validated these in vitro data by monitoring the interaction between ErCry4a and G protein in a transiently transfected neuroretinal cell line using Förster resonance energy transfer. Conclusions: Our results suggest that ErCry4a and the G protein also interact in living cells and might constitute the first biochemical signaling step in radical-pair-based magnetoreception.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Jun 27, 2022
- Source ID
- 10.3390/cells11132043
Entities
People
- Bo Leberecht
- Chad Yee
- Elmar Behrmann
- Heide Behrmann
- Henrik Mouritsen
- Jan-Oliver Voß
- Jessica Schmidt
- Jingjing Xu
- Karl-Wilhelm Koch
- Katharina Görtemaker
- Rabea Bartölke
- Vita Solovyeva
Organizations
- Air Force Office of Scientific Research
- European Research Council
- German Research Foundation