A Dense Fibrillar Collagen Scaffold Differentially Modulates Secretory Function of iPSC-Derived Vascular Smooth Muscle Cells to Promote Wound Healing
Abstract
The application of human-induced pluripotent stem cells (hiPSCs) to generate vascular smooth muscle cells (hiPSC-VSMCs) in abundance is a promising strategy for vascular regeneration. While hiPSC-VSMCs have already been utilized for tissue-engineered vascular grafts and disease modeling, there is a lack of investigations exploring their therapeutic secretory factors. The objective of this manuscript was to understand how the biophysical property of a collagen-based scaffold dictates changes in the secretory function of hiPSC-VSMCs while developing hiPSC-VSMC-based therapy for durable regenerative wound healing. We investigated the effect of collagen fibrillar density (CFD) on hiPSC-VSMC’s paracrine secretion and cytokines via the construction of varying density of collagen scaffolds. Our study demonstrated that CFD is a key scaffold property that modulates the secretory function of hiPSC-VSMCs. This study lays the foundation for developing collagen-based scaffold materials for the delivery of hiPSC-VSMCs to promote regenerative healing through guiding paracrine signaling pathways.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Apr 14, 2020
- Source ID
- 10.3390/cells9040966
Entities
People
- Alan Dardik
- Biraja C Dash
- François Berthiaume
- Hassan Peyvandi
- Henry C. Hsia
- James Nie
- Jolanta Gorecka
- Kaiti Duan
- Lara Lopes
- Ocean Setia
Organizations
- Australasian Foundation for Plastic Surgery
- National Institutes of Health Clinical Center
- United States Department of Defense