Proteomic, Metabolomic, and Lipidomic Analyses of Lung Tissue Exposed to Mustard Gas
Abstract
Sulfur mustard (HD) poses a serious threat due to its relatively simple production process. Exposure to HD in the short-term causes an inflammatory response, while long-term exposure results in DNA and RNA damage. Respiratory tract tissue models were exposed to relatively low concentrations of HD and collected at 3 and 24 h post exposure. Histology, cytokine ELISAs, and mass spectrometric-based analyses were performed. Histology and ELISA data confirmed previously seen lung damage and inflammatory markers from HD exposure. The multi-omic mass spectrometry data showed variation in proteins and metabolites associated with increased inflammation, as well as DNA and RNA damage. HD exposure causes DNA and RNA damage that results in variation of proteins and metabolites that are associated with transcription, translation and cellular energy.
Document Details
- Document Type
- Pub Defense Publication
- Publication Date
- Aug 30, 2022
- Source ID
- 10.3390/metabo12090815
Entities
People
- Allison E Clay
- Amber Prugh
- Conor Jenkins
- Daniel Angelini
- Daniel Carmany
- Elizabeth S Dhummakupt
- Gabrielle Rizzo
- Jennifer Horsmon
- Julie N Renner
Organizations
- Defense Threat Reduction Agency